We demonstrate the imaging technique for the study of marine micro-biota (e.g. size ranged from um to cm) by modifying the Fourier optics with a near IR illuminating source to improve the contrast of the high spatial frequency images and to reveal the micro-structure of the observed biological object. We also present the application of this system to the study of swimming behavior of the Artemia in free space, and the detailed structure of the spines and podia of the sea urchin. The imaging system is consisted of a collimated laser beam, a high pass Fourier optics transform system, a near IR illumination source, and a CCD digital imaging recording system. From the images recorded, we are able to track the swimming trajectory and frame- by-frame motion of Artemia, and to observe how the spines and podia of the sea urchin react to the stimulation of the environment.
Shih, Ming Chang and Hwang, Jiang-Shiou
"Modified Phase Contrast Microscopy and its Applications to the Observation of Marine Biological Objects,"
Journal of Marine Science and Technology: Vol. 8
, Article 7.
Available at: https://jmstt.ntou.edu.tw/journal/vol8/iss1/7