The fatty acid compositions and typical fuel characteristics of the biodiesels produced from Isochrysis galbana lipids and used cooking oil were analyzed and compared in this study. The experimental results showed that the major fatty acid compositions of the biodiesel made from the extracted Isochrysis galbana lipids were significantly different from those of the biodiesel made from used cooking oil. The biodiesel made from the microalgae lipids primarily comprised 35.34 wt. % myristic acid (C14:0) whereas the major compound of the used cooking-oil biodiesel was 47.51 wt. % oleic acid (C18:1). Docosahexaenoic acid (DHA) at 4.67 wt. % was only found in the microalgae biodiesel. The fuel properties of the microalgae fatty acid methyl esters were more optimal, including a significantly lower iodine number, 71.0, and higher cetane number, 65.3. Isochrysis galbana is therefore a promising feedstock source for the production of biodiesel.

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