APPLICATION OF LC-MS/MS IN IDENTIFICATION OF TOXIN IN THE CAUSATIVE GASTROPOD AND VICTIM SAMPLES

Authors

Chun-Lan Lin, Department of Food Science and Center of Excellence for the Oceans, National Taiwan Ocean University, Keelung, Taiwan, R.O.C.
Cheng-Hong Hsieh, Department of Health and Nutrition Biotechnology, Asia University, Taichung, Taiwan, R.O.C.
You-Liang Hsieh, Department of Health and Nutrition Biotechnology, Asia University, Taichung, Taiwan, R.O.C.
Yi-Cheng Tai, Department of Neurology, E-Da Hospital, Kaohsiung, Taiwan, R.O.C.
Deng-Fwu Hwang, Department of Food Science and Center of Excellence for the Oceans, National Taiwan Ocean University, Keelung, Taiwan, R.O.C. Department of Health and Nutrition Biotechnology, Asia University, Taichung, Taiwan, R.O.C.Follow

Abstract

Food paralytic poisoning incident was detected in the gastropod Nassariidae Niotha clathrata and Zeuxis scalaris occurred in December 2012 in Kaohsiung, Taiwan. The toxin was purified from each remain of cooked gastropod and victim tissues by ultrafiltration using a YM-3 membrane, followed by C18 cartridge column purification. Based on analyses by LC-MS/MS, the causative agent of gastropod food poisoning was identified as tetrodotoxin. The toxicity of remain of cooked sample was 245 MU/g (1MU = 0.178 µg TTX) for N. clathrata and 203 MU/g for Z. scalaris. The blood and excrements of victim was shown to contain TTX of 0.13 and 0.18 µg/g, respectively. There was positive between results from LC-MS/MS and mouse bioassay in the causative gastropod. The LC-MS/MS method is suitable for rapid screening of TTX.

Recommended Citation

Lin, Chun-Lan; Hsieh, Cheng-Hong; Hsieh, You-Liang; Tai, Yi-Cheng; and Hwang, Deng-Fwu (2013) "APPLICATION OF LC-MS/MS IN IDENTIFICATION OF TOXIN IN THE CAUSATIVE GASTROPOD AND VICTIM SAMPLES," Journal of Marine Science and Technology: Vol. 21: Iss. 7, Article 8.
DOI: 10.6119/JMST-013-1223-1
Available at: https://jmstt.ntou.edu.tw/journal/vol21/iss7/8