The optimum treatments of Monostroma (M.) nitidum extract were studied using response surface methodology, and pulsed microwave-assisted extraction (MAE) method was applied in this study. The study was performed using a threelevel, three-factor design and aimed at determining the optimum combinations of particle size of M. nitidum particle (X1), concentration of M. nitidum (X2), and ethanol percentage (X3). The response variables of the α-diphenyl-α-picrylhydrazyl (DPPH) radical scavenging effect and ferrous ion chelating capacity were significantly affected by particle size (X1) and the ethanol percentage (X3) at a significance level of 5%. The coefficients of determination (R2) of the response surface models of the DPPH radical scavenging effect and ferrous ion chelating capacity were found to be above 0.89. In this study the optimum treatments were established using the highest concentration of M. nitidum powder particles 1.5% with an ethanol percentage range of 10~30% and 0.38~0.25 mm particle size.
Lin, Yeu-Pyng; Wu, Shao-Chi; and Huang, Shih-Li
"EFFECTS OF MICROWAVE-ASSISTED EXTRACTION ON THE FREE RADICAL SCAVENGING AND FERROUS CHELATING ABILITIES OF MONOSTROMA NITIDUM EXTRACT,"
Journal of Marine Science and Technology: Vol. 21
, Article 15.
Available at: https://jmstt.ntou.edu.tw/journal/vol21/iss5/15