In a passive micro-scale mixer, the complete mixing of two or more fluids within a reasonable time period plays an important role. Microfluidic transportation and effective mixing are of importance and require examination. The biophysical micromixer addressed by authors would be utilized because of its excellent ability to enlarge the interface and reduce the diffusion length during the mixing process. Here, the mixing efficiency, pressure drop and Aspect ratio (AR) at an optimal inlet Reynolds ratio will be studied. Some useful results will be addressed: First, the dimensions of AX, GX, GY, FX, HX, HY and J would be effective factors when mixing the biophysical micromixer. Second, the recirculation region would play an important role in the mixing with an increment of 33.03% with respect to the original prototype because it would escalate the two-flow interaction. Lastly, the side wall effect will influence both the mixing performance and the pressure drop. An aspect ratio of AR = 10 will be suggested because it exhibits the highest mixing coefficient of 0.907 and the lowest pressure drop than previous studies cases. These findings will show the greater feasibility of the biophysical micromixer being mixed in a limited space. Additionally, these results would be useful for the mixing improvement of passive micromixer.

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