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Abstract

The integument of the squid Dosidicus gigas is an industrial waste and trouble the industrial processing. In this study, we examined the lipid composition and neuroprotective effects of D. gigas integument lipidic extract (DGI) in SH-SY5Y neuroblastoma cells which have been exposed to H2O2. The results showed that phospholipids, triacylglycerol and cholesterol accounted for 70.90 ± 4.96 (mean ± S.D.), 20.70 ± 3.54 and 8.40 ± 0.28% of DGI, respectively. And the main fatty acids were docosahexaenoic acid (30.53% of total fatty acid), palmitic acid (20.36% of total fatty acid) and eicosapentaenoic acid (11.84% of total fatty acid). DGI at the concentrations of 2, 4 and 8 mg/ml successfully protected SH-SY5Y cells against the cytotoxicity, markedly increased the cell proliferation, antioxidant enzymes of glutathione contents and catalase activities, and inhibited the secretion of amyloid β-peptide 1-42. Our results suggested that DGI may be a possible approach in the prevention of Alzheimer’s disease and other neurodegenerative diseases.

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