PURIFICATION AND CHARACTERIZATION OF A CELLULASE FROM BACILLUS SUBTILIS YJ1

Authors

Li-Jung Yin, Department of Seafood Science, National Kaohsiung Marine University, No. 142 Hai-Chuan Rd. Nan-Tzu, Kaohsiung 81143, Taiwan, R.O.CFollow
Hsin-Hung Lin, Nugen Bioscience (Taiwan) Co, Ltd. No. 29, 40th Rd., Taichung Industrial Park, Taichung 40768, Taiwan, R.O.C.
Zheng-Rong Xiao, Nugen Bioscience (Taiwan) Co, Ltd. No. 29, 40th Rd., Taichung Industrial Park, Taichung 40768, Taiwan, R.O.C.

Abstract

Broth incubated with Bacillus subtilis YJ1 at 37°C for 36 h was collected and removed bacterial cells by passing through a 0.45 µm membrane. A cellulase was purified to electrophoretical homogeneity by ammonium sulfate precipitation, Macro-Prep ion exchange and Bio-Gel P-100 chromatography. The recovery and purification fold were 9.7% and 289, respectively, after Bio-Gel P-100 chromatograph. The purified cellulase, with a molecular mass (M) of 32.5 kDa, had an optimal pH and temperature at 6.0 and 50-60°C, respectively. It was stable at pH 6.0-7.5 and

Recommended Citation

Yin, Li-Jung; Lin, Hsin-Hung; and Xiao, Zheng-Rong (2010) "PURIFICATION AND CHARACTERIZATION OF A CELLULASE FROM BACILLUS SUBTILIS YJ1," Journal of Marine Science and Technology: Vol. 18: Iss. 3, Article 19.
DOI: 10.51400/2709-6998.1895
Available at: https://jmstt.ntou.edu.tw/journal/vol18/iss3/19