The hot-water extract of seaweed Sargassum hemiphyllum was evaluated antioxidant activity by using four different methods, including DPPH free radicals scavenging activity, Fe+2 chelating activity, superoxide anion radical scavenging activity and reducing power, and the immune-stimulating activity by using HB4C5 cells and J774.1 cells. It was found that the antioxidant activity was increasing in correlating with the concentration below 3.5 mg/ml. The HB4C5 cells showed the maximum relative activities of cell proliferation (174%) and IgM secretion (132%) at 120 µg/ml of hot-water extract. Overheating slightly reduced the immune-stimulating activity of hot-water extract. At 80 µg/ml, J774.1 cells showed the maximum relative activities of cell proliferation (141%) and phagocytosis (147%). Hence, the hot-water extract of seaweed S. hemiphyllum plays the important role on antioxidant and immune-stimulating activities.
Hwang, Pai-An; Wu, Chwen-Herng; Gau, Shu-Yun; Chien, Shih-Yung; and Hwang, Deng-Fwu
"ANTIOXIDANT AND IMMUNE-STIMULATING ACTIVITIES OF HOT-WATER EXTRACT FROM SEAWEED SARGASSUM HEMIPHYLLUM,"
Journal of Marine Science and Technology: Vol. 18
, Article 5.
Available at: https://jmstt.ntou.edu.tw/journal/vol18/iss1/5